在无细胞培养基中传染性烟草花叶病毒核糖核酸的生物合成
Biosynthesis of infectious tobacco mosaic virus ribonucleic acid in a cell-free medium.
作者信息
COCHRAN G W, DHALIWAL A S, WELKIE G W, CHIDESTER J L, LEE M H, CHANDRASEKHAR B K
出版信息
Science. 1962 Oct 5;138(3536):46-8. doi: 10.1126/science.138.3536.46.
PMID:13880075
Abstract
When cell-free preparations containing large molecules and subcellular bodies extracted from tobacco-leaf cells infected with tobacco mosaic virus were incubated with MgCl(2) and the four ribonucleoside-5'-triphosphates, the level of infectivity increased about threefold in 30 minutes. The mechanism synthesizing the new infectivity was believed to be located within isolated nuclei. Ultrasonic rupture of the nuclear membranes appeared to increase the rate of synthesis.
摘要
当将含有从感染烟草花叶病毒的烟草叶细胞中提取的大分子和亚细胞体的无细胞制剂与氯化镁和四种核糖核苷 - 5'-三磷酸一起孵育时，感染性水平在30分钟内增加了约三倍。合成新感染性的机制被认为位于分离的细胞核内。核膜的超声破裂似乎提高了合成速率。
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