DVORAK H F, WAKSMAN B H
J Exp Med. 1962 Jul 1;116(1):1-16. doi: 10.1084/jem.116.1.1.
Normal Dutch rabbit lymph node and spleen minces, lymph node cell suspensions, and residues from lymph node cell suspensions were cultured in Millipore chambers with slices of autologous or homologous (New Zealand) ear skin. for varying time intervals. Lymphoid cells exposed to New Zealand ear skin for more than 4 days were found capable of producing typical "transfer reactions" in the specific New Zealand ear skin donor, similar in every way to reactions produced by cells from lymph nodes sensitized in the intact Dutch animal. Heat-killed cells and cells exposed to New Zealand ear skin for less than 4 days (in chambers or in the intact animal) or to Dutch ear skin for any period of time were incapable of eliciting such reactions. It is concluded that normal lymphoid tissues undergo primary sensitization when exposed to homografts in Millipore chambers for suitable periods of time.
将正常荷兰兔的淋巴结和脾脏切碎物、淋巴结细胞悬液以及淋巴结细胞悬液的残渣,与自体或同种(新西兰)耳皮肤切片一起在微孔培养室中培养不同的时间间隔。发现暴露于新西兰耳皮肤超过4天的淋巴细胞能够在特定的新西兰耳皮肤供体中产生典型的“转移反应”,在各方面都与在完整荷兰动物中致敏淋巴结的细胞所产生的反应相似。热灭活的细胞以及暴露于新西兰耳皮肤少于4天(在培养室或完整动物中)或暴露于荷兰耳皮肤任何时间段的细胞均无法引发此类反应。得出的结论是,正常淋巴组织在微孔培养室中暴露于同种异体移植物适当时间后会发生初次致敏。