Song G M, Wang G F, Becker W
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1992;10(2):113-6.
Oxidative phosphorylation of liver mitochondria in Oncomelania snail was separately detected by using oxygen electrode and spectrophotometer. ADP increased oxidative reaction of liver mitochondria from 0.187 to 0.318 mumol O2/mg protein.20 min. When certain substrates of citric acid cycle were added to liver mitochondria of Oncomelania snail, we found that oxidative phosphorylation increased to 0.353-0.444 mumol O2/mg protein.20 min. ATPase was detected in the liver of Oncomelania snail. The oxidative phosphorylation of mitochondria in Oncomelania snail could be markedly inhibited by DNP and molluscicide bromoacetamide, but the latter didn't show the inhibition of ATPase. (Figs. 1,2).
采用氧电极和分光光度计分别检测了钉螺肝脏线粒体的氧化磷酸化。二磷酸腺苷(ADP)使钉螺肝脏线粒体的氧化反应从0.187增加至0.318μmol O₂/(mg蛋白质·20分钟)。当向钉螺肝脏线粒体中添加某些柠檬酸循环底物时,我们发现氧化磷酸化增加至0.353 - 0.444μmol O₂/(mg蛋白质·20分钟)。在钉螺肝脏中检测到了ATP酶。2,4 - 二硝基苯酚(DNP)和杀螺剂溴乙酰胺可显著抑制钉螺线粒体的氧化磷酸化,但后者对ATP酶无抑制作用(图1、2)。