牛牙龈蛋白聚糖多功能蛋白聚糖和核心蛋白聚糖的分离与鉴定。

Isolation and characterization of bovine gingival proteoglycans versican and decorin.

Bratt P, Anderson M M, Månsson-Rahemtulla B, Stevens J W, Zhou C, Rahemtulla F

Department of Community and Public Health Dentistry, University of Alabama School of Dentistry, University Station, Birmingham 35294.

Int J Biochem. 1992 Oct;24(10):1573-83. doi: 10.1016/0020-711x(92)90173-x.

Abstract

We have isolated, chemically and immunologically characterized versican and decorin from bovine gingiva. 2. Versican was of large molecular weight and the molecular size of the core protein was estimated to be greater than 200 kDa. 3. The glycosaminoglycan chains were susceptible to chondroitinase ABC and N-linked oligosaccharides were present on the protein core of the molecule. 4. Immunological studies provided evidence that a hyaluronic acid binding region was present in the core protein of versican. 5. The overall structure was similar to that of versican isolated from bovine sclera. 6. Decorin had a molecular weight of 102 kDa and its glycosaminoglycan chain was completely digested by specific glycosidases. 7. The partially deglycosylated core protein had a molecular weight of 55 kDa and N-linked oligosaccharides were present on the molecule.

摘要

我们已从牛牙龈中分离出多功能蛋白聚糖和核心蛋白聚糖，并对其进行了化学和免疫特性分析。2. 多功能蛋白聚糖分子量较大，核心蛋白的分子大小估计大于200 kDa。3. 糖胺聚糖链对软骨素酶ABC敏感，且分子的蛋白核心上存在N-连接寡糖。4. 免疫学研究表明，多功能蛋白聚糖的核心蛋白中存在透明质酸结合区域。5. 整体结构与从牛巩膜中分离出的多功能蛋白聚糖相似。6. 核心蛋白聚糖分子量为102 kDa，其糖胺聚糖链被特定糖苷酶完全消化。7. 部分去糖基化的核心蛋白分子量为55 kDa，分子上存在N-连接寡糖。