SOLOMON J J, SAN CLEMENTE C L
Appl Microbiol. 1963 Jan;11(1):36-41. doi: 10.1128/am.11.1.36-41.1963.
A number of physiological characteristics were studied on some 29 strains of phage-propagating staphylococci belonging to the Basic International Series and the Seto-Wilson bovine-adapted set. All the cultures except strain 73 were coagulase-positive, with reciprocal titers ranging from 2 to 8,192. Strain 73 was again an exception with respect to phosphatase activity. Group 1 yielded high values for both phosphatase and oxygen uptake but low values for extracellular protein. Resistance to penicillin was demonstrated only by strains 80, 81, 53, 54, 75, and 77. Strain 70, one of the highest coagulase producers, alone showed no catalase activity. Mannitol was fermented by all coagulase-positive strains. Hemolysis of one or more of three kinds of erythrocytes (sheep, rabbit, and human) was a common characteristic of most strains. However, pigmentation was a nondiscriminating parameter. Although one-half of the cultures liquefied gelatin, most of them gave similar antibiotic-sensitivity tests, except the six which were penicillinase producers. There was little difference in growth rate for all strains. Comparison of coagulase production to cell size indicated that the high-titer strains were generally larger than the low producers. The foregoing evidence avers that, in addition to lytic spectrum, physiological properties can usefully characterize staphylococcal phage-propagating strains.
对属于基础国际系列和濑户 - 威尔逊牛适应株系的约29株噬菌体增殖葡萄球菌的一些生理特性进行了研究。除73株外,所有培养物的凝固酶均呈阳性,效价范围为2至8192。73株在磷酸酶活性方面再次成为例外。第1组的磷酸酶和氧摄取值较高,但细胞外蛋白值较低。仅80、81、53、54、75和77株对青霉素有抗性。70株是最高的凝固酶产生菌之一,单独显示无过氧化氢酶活性。所有凝固酶阳性菌株均发酵甘露醇。三种红细胞(绵羊、兔子和人类)中的一种或多种发生溶血是大多数菌株的共同特征。然而,色素沉着是一个无鉴别力的参数。虽然一半的培养物能液化明胶,但除了6株产青霉素酶的菌株外,大多数菌株的抗生素敏感性试验结果相似。所有菌株的生长速率差异不大。凝固酶产生量与细胞大小的比较表明,高滴度菌株通常比低产生菌大。上述证据表明,除裂解谱外,生理特性也可有效地用于表征葡萄球菌噬菌体增殖菌株。