Riparbelli M G, Callaini G
Department of Evolutionary Biology, University of Siena, Italy.
J Cell Sci. 1992 Jun;102 ( Pt 2):299-305. doi: 10.1242/jcs.102.2.299.
The changing distribution of a nuclear envelope antigen recognized by a monoclonal antibody raised against human fibroblast vimentin during the syncytial mitoses of the Drosophila embryo has been studied with a confocal laser scanning microscope. The antigen appears very early as irregular aggregates in the peripheral cytoplasm of the preblastoderm embryo. As the first nuclei reach the periplasm the antigen is localized on the nuclear envelope and the cytoplasmic staining decreases. In addition to the perinuclear labeling we observed intense midzone and polar staining during the mitotic cycle. A possible relationship between polar localization of the antigen and centrosome position is discussed.
利用共聚焦激光扫描显微镜研究了在果蝇胚胎的合胞体有丝分裂过程中，一种由针对人成纤维细胞波形蛋白产生的单克隆抗体识别的核膜抗原的分布变化。该抗原最早以不规则聚集体的形式出现在胚盘前胚胎的外周细胞质中。当第一批细胞核到达周质时，抗原定位于核膜上，细胞质染色减少。除了核周标记外，我们在有丝分裂周期中还观察到强烈的中间区和极区染色。讨论了抗原的极区定位与中心体位置之间的可能关系。
