用高效液相色谱法定量测定犬和大鼠血浆中一种新型胆囊收缩素和胃泌素受体拮抗剂（L-365,260）。

Quantitation of a new cholecystokinin and gastrin receptor antagonist (L-365,260) in dog and rat plasma by high-performance liquid chromatography.

作者信息

Chen I W, Lin J H, Dorley J

机构信息

Merck Sharp & Dohme Research Laboratories, West Point, PA 19486.

出版信息

J Chromatogr. 1992 May 8;576(2):363-7. doi: 10.1016/0378-4347(92)80213-a.

DOI:10.1016/0378-4347(92)80213-a

PMID:1400726

Abstract

A high-performance liquid chromatographic (HPLC) procedure has been developed for the quantification of L-365,260 (I), a cholecystokinin and gastrin receptor antagonist, in dog and rat plasma. The method involves liquid-liquid extraction and HPLC with ultraviolet detection. Standard curves were linear over the range 7.5-2000 ng/ml for rat and dog plasma. The method is reproducible and reliable with a detection limit of 7.5 ng/ml in biological fluids. The mean coefficients of variation for concentrations within the range of the standard curve range were 3.84 and 2.56%, respectively, for intra-day analysis and 4.48 and 4.26%, respectively, for inter-day analysis. Application of the development was successfully demonstrated by quantifying the concentration of I in both dog and rat plasma samples following an intravenous or oral dose of 5 mg/kg I.

摘要

已开发出一种高效液相色谱（HPLC）方法，用于定量测定犬和大鼠血浆中的L-365,260（I），一种胆囊收缩素和胃泌素受体拮抗剂。该方法包括液-液萃取和带紫外检测的HPLC。大鼠和犬血浆的标准曲线在7.5-2000 ng/ml范围内呈线性。该方法具有可重复性和可靠性，生物流体中的检测限为7.5 ng/ml。标准曲线范围内浓度的日内分析平均变异系数分别为3.84%和2.56%，日间分析分别为4.48%和4.26%。通过定量静脉或口服5 mg/kg I后犬和大鼠血浆样品中I的浓度，成功证明了该方法的应用。