[肺炎衣原体对Hep-2细胞和HL细胞敏感性的比较]

[Comparison of sensitivity of Hep-2 cells with that of HL cells against Chlamydia pneumoniae].

作者信息

Yoshizawa H, Dairiki K, Yamazaki T

机构信息

Department of Pathobiology, School of Nursing, Chiba University.

出版信息

Kansenshogaku Zasshi. 1992 Aug;66(8):1037-41. doi: 10.11150/kansenshogakuzasshi1970.66.1037.

DOI:10.11150/kansenshogakuzasshi1970.66.1037

PMID:1402106

Abstract

We compared the growth of Chlamydia pneumoniae, reference strain TW183 and an isolate from a Japanese infant, AC43 in HeLa229, HL and Hep-2 cells. The mean number of inclusion-forming units was significantly higher on HL cells than HeLa229 cells, when the cells were not pretreated with DEAE-dextran. When the cells were not pretreated with DEAE-dextran, Hep-2 cells had a higher mean number of inclusion-forming units and a higher yield than other cell lines. Iscove's modified Dulbecco medium enhanced growth of strain TW183 in HeLa229 cells but had a low yield of strain AC43 in Hep-2 cells.

摘要

我们比较了肺炎衣原体参考菌株TW183和一株来自日本婴儿的分离株AC43在HeLa229、HL和Hep-2细胞中的生长情况。当细胞未用DEAE-葡聚糖预处理时，HL细胞上形成包涵体的单位平均数显著高于HeLa229细胞。当细胞未用DEAE-葡聚糖预处理时，Hep-2细胞的形成包涵体单位平均数和产量高于其他细胞系。Iscove改良的杜尔贝科培养基可促进TW183菌株在HeLa229细胞中的生长，但在Hep-2细胞中AC43菌株的产量较低。