HURLBUT W P
J Gen Physiol. 1963 Jul;46(6):1223-48. doi: 10.1085/jgp.46.6.1223.
Desheathed frog (R. pipiens) sciatic nerves were soaked in Na-deficient solutions, and measurements were made of their Na and K contents and of the movements of K(42). When a nerve is in Ringer's solution, the Na fluxes are equal to the K fluxes, and about 75 per cent of the K influx is due to active transport. The Na content and the Na efflux are linearly related to the Na concentration of the bathing solution, while the K content and the K fluxes are not so related. When a nerve is in a solution in which 75 per cent of the NaCl has been replaced by choline chloride or sucrose, the active K influx exceeds the active Na efflux, and the K content is maintained. When a nerve is soaked in a solution that contains Li, the K(42) uptake is inhibited, and the nerve loses K and gains Li. When a Li-loaded nerve recovers in a Li-free solution, K is taken up in exchange for Li. This uptake of K requires Na in the external solution. It is concluded that the active transports of K and of Na may be due to different processes, that an accumulation of K occurs only in exchange for an intracellular cation, which need not be Na, and that Na plays a specific, but unknown, role in K transport.
剥除髓鞘的牛蛙（豹蛙）坐骨神经浸泡于缺钠溶液中，对其钠和钾含量以及钾（42）的移动进行了测量。当神经处于任氏液中时，钠通量与钾通量相等，且约75%的钾内流是由主动转运引起的。钠含量和钠外流与浸泡溶液的钠浓度呈线性关系，而钾含量和钾通量则无此关系。当神经处于75%的氯化钠被氯化胆碱或蔗糖替代的溶液中时，主动钾内流超过主动钠外流，且钾含量得以维持。当神经浸泡于含锂溶液中时，钾（42）摄取受到抑制，神经失钾而得锂。当负载锂的神经在无锂溶液中恢复时，钾被摄取以交换锂。这种钾的摄取需要细胞外溶液中有钠。得出的结论是，钾和钠的主动转运可能归因于不同过程，钾的积累仅在与一种细胞内阳离子交换时发生，该阳离子不一定是钠，且钠在钾转运中起特定但未知的作用。
