Howard S, Anderson C, Diels W, Gerres K, Garcia B
Department of Pathology, University of Western Ontario, London, Canada.
Pathol Res Pract. 1992 Jun;188(4-5):497-9. doi: 10.1016/S0344-0338(11)80044-0.
Nuclear DNA density analysis was carried out on six normal control human parathyroid glands and forty-six pathological lesions of human parathyroid tissue. The pathological glands had originally been classified as twenty hyperplasias, twenty-one adenomas, and five carcinomas in accordance with clinical-pathological information. Specimens had been obtained through the Surgical Pathology Department of the University Hospital, London, in the form of either imprints from the cut surface of a lesion, or as cell separation samples from a paraffin block. Nuclear DNA ploidy analysis of the normal glands showed, as expected, a diploid cell population. Sixty-one percent of nuclei of the carcinomas were above 2C, extending to 7C. There was a significant difference in nuclear DNA content between normal and benign tissue versus tissue classified as carcinoma. No significant difference in nuclear DNA ploidy could be established between the benign adenoma and hyperplasia tissue; however, marked significant DNA ploidy differences were found between tissue from the latter two and carcinoma. DNA ploidy from cell image analysis using Feulgen's reaction can be useful in establishing a differential diagnosis between carcinoma and benign lesions.
对六个正常对照人甲状旁腺以及四十六个甲状旁腺组织病理病变进行了核DNA密度分析。根据临床病理信息，这些病理腺体最初被分类为二十个增生、二十一个腺瘤和五个癌。标本是通过伦敦大学医院外科病理科获得的，形式为病变切面的印片，或石蜡块中的细胞分离样本。正常腺体的核DNA倍性分析显示，正如预期的那样，是二倍体细胞群。61%的癌细胞核高于2C，延伸至7C。正常和良性组织与分类为癌的组织之间的核DNA含量存在显著差异。良性腺瘤和增生组织之间在核DNA倍性上没有显著差异；然而，在后两者与癌的组织之间发现了明显的显著DNA倍性差异。使用福尔根反应的细胞图像分析得出的DNA倍性，在建立癌与良性病变的鉴别诊断中可能有用。
