PEACHEY L D
J Cell Biol. 1964 Jan;20(1):95-111. doi: 10.1083/jcb.20.1.95.
Electron microscopic evidence is presented, from mitochondria in whole cells of toad urinary bladder and from isolated rat kidney mitochondria, indicating that the divalent cations calcium, strontium, and barium are accumulated in granules localized in the mitochondrial matrix. This accumulation occurs under conditions in which divalent ions are present in the medium bathing either whole cells or isolated mitochondria. The evidence indicates that the divalent ions are deposited on, or in a pre-existing granule, possibly in exchange for other ions. It suggests a possible role of the intramitochondrial granules in the regulation of the internal ionic environment of the mitochondrion. Certain biochemical and physiological implications of this phenomenon are discussed.
本文提供了电子显微镜证据,这些证据来自蟾蜍膀胱全细胞中的线粒体以及分离的大鼠肾线粒体,表明二价阳离子钙、锶和钡在位于线粒体基质中的颗粒中积累。这种积累发生在培养基中存在二价离子的条件下,该培养基用于培养全细胞或分离的线粒体。证据表明二价离子沉积在预先存在的颗粒上或颗粒内部,可能是与其他离子进行交换。这表明线粒体内颗粒在调节线粒体内部离子环境方面可能发挥作用。本文还讨论了这一现象的某些生化和生理意义。