LAU H S, WISEMAN R F
J Bacteriol. 1964 Feb;87(2):337-40. doi: 10.1128/jb.87.2.337-340.1964.
Lau, H. S. (University of Kentucky, Lexington), and R. F. Wiseman. Effect of uric acid on riboflavine production by intestinal coliform bacteria. J. Bacteriol. 87:337-340. 1964.-Cultures of Escherichia coli, Aerobacter aerogenes, and Paracolobactrum species, isolated from human feces and from intestinal contents of rats, were tested for riboflavine synthesis in a chemically defined medium. Riboflavine was assayed by a fluorometric method and by microbiological assay. When uric acid was added to the synthetic medium at levels ranging from 10 to 750 mug/ml, it inhibited the production of riboflavine, but did not affect the growth of the bacteria. As the amount of uric acid in the medium was increased from 10 to 750 mug, there was a corresponding decrease in the amount of riboflavine produced. Some disappearance of uric acid was observed in the cultures. These results suggest that uric acid, or its degradation products, may have acted as an antimetabolite or an enzyme inactivator in the biosynthesis of riboflavine.
刘,H. S.(肯塔基大学,列克星敦)和R. F. 怀斯曼。尿酸对肠道大肠菌产生核黄素的影响。《细菌学杂志》87:337 - 340。1964年。——从人类粪便和大鼠肠道内容物中分离出的大肠杆菌、产气气杆菌和副大肠杆菌菌株,在化学限定培养基中进行核黄素合成测试。核黄素通过荧光法和微生物测定法进行测定。当尿酸以10至750微克/毫升的浓度添加到合成培养基中时,它会抑制核黄素的产生,但不影响细菌的生长。随着培养基中尿酸量从10微克增加到750微克,产生的核黄素量相应减少。在培养物中观察到尿酸有一些消失。这些结果表明,尿酸或其降解产物可能在核黄素的生物合成中充当抗代谢物或酶失活剂。