INOUE Y K
Bull World Health Organ. 1964;30(2):181-5.
In the course of studies towards the development of a live, attenuated Japanese encephalitis vaccine, an attenuated mutant (m mutant) of Japanese encephalitis virus was isolated after serial passages of a parental virus, Mukai strain, in embryonic mouse skin cultures. The low virulence of the m mutant was found to be stable in tissue cultures but reversion to virulence was observed after multiplication of the virus in mouse brain. The m mutant induced high immunity in mice after vaccination in a single intraperitoneal injection. Furthermore, a high virus yield was obtained from the m mutant in tissue culture, with titres of about 10(9.5) TCD(50) per ml. It is suggested that this mutant may be suitable for the preparation of inactivated tissue culture vaccine, but the author stresses that it cannot be recommended for tests in man until more is known about its genetic stability.
在研发减毒活日本脑炎疫苗的研究过程中,通过亲本病毒向井株在胚胎小鼠皮肤培养物中连续传代后,分离出了一种日本脑炎病毒的减毒突变株(m突变株)。发现m突变株在组织培养中的低毒力是稳定的,但在小鼠脑内病毒增殖后观察到毒力回复。m突变株经单次腹腔注射免疫小鼠后可诱导产生高免疫力。此外,m突变株在组织培养中可获得高病毒产量,每毫升滴度约为10(9.5) TCD(50)。有人认为该突变株可能适合制备灭活组织培养疫苗,但作者强调,在对其遗传稳定性有更多了解之前,不能推荐用于人体试验。