尿苷-C14掺入L细胞RNA的动力学
KINETICS OF INCORPORATION OF URIDINE-C14 INTO L CELL RNA.
作者信息
RAKE A V, GRAHAM A F
出版信息
Biophys J. 1964 Jul;4(4):267-84. doi: 10.1016/s0006-3495(64)86782-5.
Abstract
Five components have been isolated from L cells by a combination of phenol extraction procedures and sedimentation analysis through sucrose gradients. These components are identified by their sedimentation rates. The 50S and 40S components are derived from the nucleus, the 32S and 18S from ribosomal RNA, and the 4S fraction is the soluble RNA of the cell. L cells were supplied with uridine-C(14) under steady-state conditions and the rate of uptake of C(14) into each component was measured. Analysis of the results suggests that the delay in entry of C(14) into ribosomal RNA is occasioned by two sequential precursors and that 50S and 40S RNA meet the kinetic requirements for these precursors. 4S RNA seems to contain two components that label at different rates.
摘要
通过酚提取程序和蔗糖梯度沉降分析相结合的方法，从L细胞中分离出了五种成分。这些成分通过它们的沉降速率来鉴定。50S和40S成分来自细胞核，32S和18S来自核糖体RNA，4S部分是细胞的可溶性RNA。在稳态条件下给L细胞提供尿苷-C(14)，并测量C(14)进入每种成分的摄取速率。对结果的分析表明，C(14)进入核糖体RNA的延迟是由两个连续的前体引起的，并且50S和40S RNA符合这些前体的动力学要求。4S RNA似乎包含两种以不同速率标记的成分。
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