用培养的组织细胞和抗代谢物测定维生素和氨基酸

ASSAY OF VITAMINS AND AMINO ACIDS WITH CULTURED TISSUE CELLS AND ANTIMETABOLITES.

作者信息

SAVCHUCK W B, MERRIMAN M E, LOCKHART W L

出版信息

Appl Microbiol. 1964 Jul;12(4):316-20. doi: 10.1128/am.12.4.316-320.1964.

DOI:10.1128/am.12.4.316-320.1964

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1058124/

Abstract

A survey of growth responses of tissue-culture cells to vitamins and amino acids was undertaken to explore the potentialities of tissue culture in the assay of growth factors. An antagonist of the nutrient was included in each test system to improve its sensitivity. Addition of an antimetabolite was advantageous in the thiamine and phenylalanine assays. Tissue-culture assays of tryptophan and of phenylalanine supplemented with beta-2-thienylalanine compared favorably with microbial assays, and may serve as confirmatory or supplementary test systems. The sensitivity of cultured tissue cells to minute amounts of a variety of physiologically active substances suggests their employment in hormone and toxic compound assays.

摘要

为了探索组织培养在生长因子测定中的潜力，开展了一项关于组织培养细胞对维生素和氨基酸生长反应的调查。每个测试系统中都包含一种营养素拮抗剂，以提高其灵敏度。在硫胺素和苯丙氨酸测定中添加抗代谢物是有利的。用β-2-噻吩丙氨酸补充的色氨酸和苯丙氨酸的组织培养测定与微生物测定相比具有优势，可作为确证性或补充性测试系统。培养的组织细胞对微量多种生理活性物质的敏感性表明它们可用于激素和有毒化合物的测定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/060d/1058124/b8b97d2d12a9/applmicro00354-0032-a.jpg

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