Ofosu-Appiah W, Ruggiero C
Masonic Medical Research Laboratory, Utica, New York 13501.
Cell Immunol. 1992 Nov;145(1):130-45. doi: 10.1016/0008-8749(92)90318-j.
Suppressor T cell function in the spontaneously hypertensive rat (SHR) and normotensive Wistar Kyoto (WKY) rats was analyzed using syngeneic mixed lymphocyte reaction (SMLR) and concanavalin A (Con A) activation. A depressed SMLR was found in adult SHR but not in adult WKY. IL-2 synthesized by SHR was 40-fold lower than that of WKY, and the suppressor T cells generated in the SMLR were incapable of suppressing IgG synthesis. Precursors of cells that can be activated by Con A to become functional suppressor cells are reduced in adult SHR. Supernatant fluids derived from Con A-activated spleen cells from adult SHR failed to significantly inhibit IgG synthesis by cultures of syngeneic spleen cells compared to supernatant fluids from young SHR or WKY Con A-activated spleen cells. However, spleen cells from both adult SHR and WKY proliferated strongly and released equivalent amounts of IL-2 in response to Con A. Addition of exogenous IL-2 to the SMLR cultures in vitro restored the ability of SHR T cells to respond in the SMLR, with generation of cells capable of suppressing IgG synthesis. Administration of SHR with IL-2 in vivo also restored the suppressor T cell function in the SMLR. These results suggest a defective suppressor T cell activation and loss of suppressor T cell activity as the SHR age.
利用同基因混合淋巴细胞反应（SMLR）和伴刀豆球蛋白A（Con A）激活，分析自发性高血压大鼠（SHR）和正常血压的Wistar Kyoto（WKY）大鼠的抑制性T细胞功能。在成年SHR中发现SMLR受到抑制，但成年WKY中未出现这种情况。SHR合成的白细胞介素-2（IL-2）比WKY低40倍，且SMLR中产生的抑制性T细胞无法抑制IgG合成。在成年SHR中，可被Con A激活成为功能性抑制细胞的细胞前体减少。与幼年SHR或WKY的Con A激活脾细胞的上清液相比，成年SHR的Con A激活脾细胞的上清液未能显著抑制同基因脾细胞培养物中的IgG合成。然而，成年SHR和WKY的脾细胞对Con A均有强烈增殖反应，并释放等量的IL-2。在体外向SMLR培养物中添加外源性IL-2可恢复SHR T细胞在SMLR中的反应能力，并产生能够抑制IgG合成的细胞。在体内给SHR注射IL-2也可恢复SMLR中的抑制性T细胞功能。这些结果表明，随着SHR年龄增长，抑制性T细胞激活存在缺陷且抑制性T细胞活性丧失。
