DAS C C, KAUFMANN B P, GAY H
J Cell Biol. 1964 Dec;23(3):423-30. doi: 10.1083/jcb.23.3.423.
Employing cytochemical methods it was found that during the early embryonic development of Drosophila melanogaster the nuclei contain in sequence two kinds of chromosomal proteins. The cleavage nuclei (as also the pronuclei), until shortly before the blastoderm stage, contain an atypical (or juvenile) histone, stainable with bromophenol blue but not with alkaline fast green. The typical fast green-positive histone appears at the close of the period of the synchronized cleavage mitoses, just before blastulation, when nucleoli are first produced. The amount of DNA of the cleavage nuclei, as determined cytophotometrically, is nearly constant; therefore, the DNA moiety of the nucleohistone complex seems to remain unaffected by the protein shift during embryonic development. The implications of the protein shift in relation to the histone control of gene expression are discussed.
运用细胞化学方法发现,在黑腹果蝇的早期胚胎发育过程中,细胞核依次含有两种染色体蛋白。直到囊胚层阶段前不久,卵裂核(以及原核)都含有一种非典型(或幼稚型)组蛋白,这种组蛋白可用溴酚蓝染色,但不能用碱性固绿染色。典型的固绿阳性组蛋白在同步卵裂有丝分裂期即将结束时,就在囊胚形成前首次产生核仁时出现。通过细胞光度测定法测定,卵裂核的DNA量几乎是恒定的;因此,核组蛋白复合物中的DNA部分在胚胎发育过程中似乎不受蛋白质变化的影响。文中讨论了蛋白质变化与组蛋白对基因表达的控制之间的关系。