移植免疫的定量分析。I. 方法。

NAJARIAN J S, FELDMAN J D

J Exp Med. 1965 Apr 1;121(4):521-31. doi: 10.1084/jem.121.4.521.

A technique for in vivo quantitation of transplantation immunity has been presented. Known numbers of H(3)-thymidine-labeled lymphoid cells were injected intravenously into isologous or homologous recipients. The total radioactivity in host spleens was counted at different time intervals. The rate of isotope disappearance from the spleen was exponential to 0.5 per cent of the dose or less. In isologous recipients, the calculated half-life of labeled cells was 82 hours; in homologous recipients it was 40 hours; and in presensitized homologous hosts it was 18 hours. The technique is a highly reproducible, precise, and sensitive measure of transplantation immunity.

已提出一种体内定量移植免疫的技术。将已知数量的H(3)-胸苷标记的淋巴细胞静脉注射到同基因或异基因受体中。在不同时间间隔对宿主脾脏中的总放射性进行计数。脾脏中同位素消失的速率呈指数下降，降至剂量的0.5%或更低。在同基因受体中，标记细胞的计算半衰期为82小时；在异基因受体中为40小时；在预先致敏的异基因宿主中为18小时。该技术是一种高度可重复、精确且灵敏的移植免疫测量方法。