Feldman S T, Gately D, Schönthal A, Feramisco J R
Department of Ophthalmology, Theodore Gildred Cancer Center, University of California, San Diego, La Jolla 92093.
Invest Ophthalmol Vis Sci. 1992 Nov;33(12):3307-14.
The authors evaluated the effects of stimulation (by serum, wounding, and three peptide growth factors: fibroblast growth factor [FGF], insulin, and transforming growth factor-beta [TGF-beta 1]) on the expression of the protein product of the immediate early gene, c-fos in bovine corneal endothelial (BCE) cells. These results were compared with those of cells which were made quiescent by serum starvation. They also examined the effect of these same growth factors or wounding on DNA synthesis. Quiescent cells expressed low levels of c-fos protein. Serum was the most potent stimulator, whereas FGF and insulin were modest stimulators. TGF-beta 1 did not significantly stimulate c-fos protein production. The results from DNA synthesis were different. Serum and FGF were still the most potent stimulators; insulin and TGF-beta 1 were weak stimulators. These data suggest that growth factors induce c-fos protein in BCE cells and that this may in part regulate the downstream event, cellular proliferation. Further investigation into the regulation of this and other protooncogene products may provide insight into the mechanisms which modulate corneal endothelial cell growth in humans.
作者评估了刺激因素(血清、创伤以及三种肽生长因子:成纤维细胞生长因子[FGF]、胰岛素和转化生长因子-β[TGF-β1])对牛角膜内皮(BCE)细胞中即刻早期基因c-fos蛋白产物表达的影响。将这些结果与通过血清饥饿处理而进入静止状态的细胞的结果进行了比较。他们还研究了这些相同的生长因子或创伤对DNA合成的影响。静止细胞表达低水平的c-fos蛋白。血清是最有效的刺激物,而FGF和胰岛素是中等刺激物。TGF-β1并未显著刺激c-fos蛋白的产生。DNA合成的结果则不同。血清和FGF仍然是最有效的刺激物;胰岛素和TGF-β1是较弱的刺激物。这些数据表明生长因子可诱导BCE细胞中的c-fos蛋白,并且这可能部分调节下游事件——细胞增殖。对这种及其他原癌基因产物调控的进一步研究可能会为调节人类角膜内皮细胞生长的机制提供深入了解。