转化DNA的亚硝酸突变：作用方式的考量

NITROUS ACID MUTATION OF TRANSFORMING DNA: CONSIDERATION OF MODE OF ACTION.

作者信息

GOODGAL S H, POSTEL E H

出版信息

Science. 1965 May 21;148(3673):1095-7. doi: 10.1126/science.148.3673.1095.

DOI:10.1126/science.148.3673.1095

Abstract

Apparently nitrous acid produces genetic alterations, expressed as antibiotic-resistant markers, directly on heat-denatured transforming DNA of Haemophilus influenzae, rather than producing DNA which acts as a non-specific mutagen. The markers which arise as a result of treatment with nitrous acid behave similarly to naturally occurring antibiotic markers. In addition, data comparing the expression and replication of induced markers to natural markers suggest that the nitrous acid-induced markers express and multiply in the same fashion as do "normal" markers. Therefore, mutations which require additional time to produce a functional DNA by a base-pair substitution, or by replication of the introduced DNA, are not responsible for the mutants observed.

摘要

显然，亚硝酸直接作用于流感嗜血杆菌经热变性的转化DNA上，产生以抗抗生素标记形式表现的基因改变，而不是产生作为非特异性诱变剂的DNA。用亚硝酸处理后出现的标记与天然存在的抗抗生素标记表现相似。此外，将诱导标记与天然标记的表达和复制进行比较的数据表明，亚硝酸诱导的标记与“正常”标记以相同方式表达和增殖。因此，通过碱基对替换或通过导入DNA的复制需要额外时间来产生功能性DNA的突变，并非所观察到的突变体产生的原因。