Chung T K, Baker D H
Department of Animal Sciences, University of Illinois, Urbana 61801.
J Anim Sci. 1992 Oct;70(10):3102-11. doi: 10.2527/1992.70103102x.
Two growth assays and one nitrogen balance experiment were conducted to develop an ideal amino acid pattern for 10-kg pigs. Crossbred pigs were fed chemically defined amino acid diets containing four indispensable amino acid (AA) patterns: 1) the Illinois final amino acid pattern (IFP), a recently developed AA profile for purified diets; 2) the Illinois ideal amino acid pattern (IIP), a modification of IFP; 3) the Wang and Fuller ideal amino acid pattern (WFIP); and 4) the 1988 National Research Council (NRC) amino acid requirement pattern for 10-kg pigs (NRCP). A mixture of dispensable AA consisting of glutamate, glycine, and proline that had been proven to be an efficient mixture of dispensable AA nitrogen was fed together with the indispensable AA patterns. Diets were made isonitrogenous and isoenergetic within experiments. In Exp. 1, pigs were given ad libitum access to experimental diets with AA levels set above the NRC AA requirements. Regardless of which AA pattern was fed, pigs had similar (P greater than .05) daily gains, daily feed intakes, and gain:feed ratios. In Exp. 2, all levels of indispensable and dispensable AA were reduced to 50% of levels present in Exp. 1. When pigs had ad libitum access to these diets, daily gains of pigs fed IIP were superior (P greater than .05) to those of pigs fed IFP or NRCP, but similar (P greater than .05) weight gains occurred in pigs fed IFP, WFIP, and NRCP. In Exp. 3, the efficiency of nitrogen utilization of the four indispensable AA patterns was evaluated by a nitrogen balance experiment in pigs equally fed the same experimental diets fed in Exp. 2. Pigs fed NRCP utilized nitrogen with an efficiency of 74%, which was less (P less than .001) than the efficiencies of 79 to 80% obtained in pigs fed IFP, IIP, and WFIP. Nitrogen retained (grams) per gram of nitrogen intake from indispensable AA was greater (P less than .01) for IIP than for either IFP or WFIP. The results of these experiments indicate that WFIP contains excesses of leucine, valine, phenylalanine plus tyrosine, methionine plus cystine, and threonine for pigs between 10 and 20 kg BW. Also, NRCP is probably first-limiting in leucine and also limiting in other AA, resulting in lower nitrogen utilization than IIP. The pattern of indispensable AA in IIP (grams of AA/100 g lysine) is as follows: lysine (100), methionine+cystine (60), threonine (65), tryptophan (18), phenylalanine+tyrosine (95), leucine (100), isoleucine (60), valine (68), arginine (42), and histidine (32).
进行了两项生长试验和一项氮平衡实验,以确定10千克体重仔猪的理想氨基酸模式。选用杂交仔猪,饲喂含有四种必需氨基酸(AA)模式的化学合成氨基酸日粮:1)伊利诺伊最终氨基酸模式(IFP),一种最近为纯化日粮开发的氨基酸谱;2)伊利诺伊理想氨基酸模式(IIP),IFP的改良版;3)王和富勒理想氨基酸模式(WFIP);4)1988年美国国家研究委员会(NRC)10千克体重仔猪的氨基酸需求模式(NRCP)。将由谷氨酸、甘氨酸和脯氨酸组成的非必需氨基酸混合物与必需氨基酸模式一起饲喂,该混合物已被证明是一种高效的非必需氨基酸氮混合物。在实验中,日粮保持等氮和等能量。在实验1中,给仔猪自由采食高于NRC氨基酸需求量的实验日粮。无论饲喂哪种氨基酸模式,仔猪的日增重、日采食量和增重:采食量比均相似(P>0.05)。在实验2中,所有必需和非必需氨基酸水平降至实验1中水平的50%。当仔猪自由采食这些日粮时,饲喂IIP的仔猪日增重优于(P>0.05)饲喂IFP或NRCP的仔猪,但饲喂IFP、WFIP和NRCP的仔猪体重增加相似(P>0.05)。在实验3中,通过氮平衡实验评估了四种必需氨基酸模式在仔猪体内的氮利用效率,这些仔猪等量饲喂与实验2相同的实验日粮。饲喂NRCP的仔猪氮利用率为74%,低于(P<0.001)饲喂IFP、IIP和WFIP的仔猪所获得的79%至80%的利用率。每克来自必需氨基酸的氮摄入量中,IIP保留的氮(克)比IFP或WFIP更多(P<0.01)。这些实验结果表明,对于体重在10至20千克的仔猪,WFIP中亮氨酸、缬氨酸、苯丙氨酸加酪氨酸、蛋氨酸加胱氨酸和苏氨酸含量过高。此外,NRCP可能首先在亮氨酸上受限,在其他氨基酸上也受限,导致氮利用率低于IIP。IIP中必需氨基酸模式(每100克赖氨酸中氨基酸的克数)如下:赖氨酸(100)、蛋氨酸+胱氨酸(60)、苏氨酸(65)、色氨酸(18)、苯丙氨酸+酪氨酸(95)、亮氨酸(100)、异亮氨酸(60)、缬氨酸(68)、精氨酸(42)和组氨酸(32)。