de las Heras M A, Gonzalez S I, Calandra R S
Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
J Reprod Fertil. 1992 Sep;96(1):323-30. doi: 10.1530/jrf.0.0960323.
Unilateral orchidectomy resulted in a significant decrease in tissue content of putrescine and polyamines. However, no differences were detected when the results were expressed in terms of ng g-1 tissue. At 48 h after bilateral orchidectomy, a significant decrease in putrescine content was observed, but spermidine and spermine content were unaffected. The observed decrease in putrescine was prevented by treatment with testosterone propionate, but neither spermidine nor spermine were affected. Bilateral orchidectomy resulted in a significant decrease in the tissue content of putrescine, spermidine and spermine after 7 days. Treatment with testosterone propionate increased the content of putrescine, spermidine and spermine in the epididymis by about 200%, 92% and 34%, respectively. When results were expressed as nmol g-1, a significant decrease after castration in putrescine and spermidine, but not in spermine, was observed. Treatment with testosterone propionate restored putrescine concentration, but had no effect on spermidine and spermine concentrations. In castrated rats treated with testosterone propionate, the anti-androgen flutamide abolished the effect of the androgen on putrescine and spermidine content, but there was no effect on spermine. Acetylputrescine was not detected in the epididymis, while acetylpolyamines were detected at much lower concentrations than polyamines. After bilateral orchidectomy there was a decrease in the tissue content of all acetylpolyamines and an increase in their tissue concentration. The effect of castration on acetylpolyamine content was reversed by testosterone propionate treatment. We conclude that an active synthesis of polyamines occurs in the rat epididymis, and that this process depends upon the androgen environment. Regulation of ornithine decarboxylase activity appears to be the main step that is controlled by androgens.
单侧睾丸切除术导致腐胺和多胺的组织含量显著降低。然而，当结果以每克组织纳克表示时，未检测到差异。双侧睾丸切除术后48小时，观察到腐胺含量显著降低，但亚精胺和精胺含量未受影响。丙酸睾酮治疗可防止观察到的腐胺减少，但亚精胺和精胺均未受影响。双侧睾丸切除术后7天，腐胺、亚精胺和精胺的组织含量显著降低。丙酸睾酮治疗使附睾中腐胺、亚精胺和精胺的含量分别增加了约200%、92%和34%。当结果以每克组织纳摩尔表示时，去势后腐胺和亚精胺显著降低，但精胺未降低。丙酸睾酮治疗可恢复腐胺浓度，但对亚精胺和精胺浓度无影响。在用丙酸睾酮治疗的去势大鼠中，抗雄激素氟他胺消除了雄激素对腐胺和亚精胺含量的影响，但对精胺无影响。附睾中未检测到乙酰腐胺，而检测到的乙酰多胺浓度远低于多胺。双侧睾丸切除术后，所有乙酰多胺的组织含量降低，其组织浓度增加。丙酸睾酮治疗可逆转去势对乙酰多胺含量的影响。我们得出结论，大鼠附睾中存在多胺的活跃合成，且这一过程取决于雄激素环境。鸟氨酸脱羧酶活性的调节似乎是受雄激素控制的主要步骤。
