对福布斯海盘车幽门盲囊脂质的化学和组织化学研究。
A chemical and histochemical study of the lipides of the pyloric cecum of the starfish, Asterias forbesi.
作者信息
KARNOVSKY M L, JEFFREY S S, THOMPSON M S, DEANE H W
出版信息
J Biophys Biochem Cytol. 1955 Mar;1(2):173-82. doi: 10.1083/jcb.1.2.173.
The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means. Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, and aldehyde lipides. The results of the histochemical study were in good agreement with the chemical data as to the nature of the phosphatide fraction, the presence of acetone-soluble aldehyde lipides, and the composition of the free droplet fat. Homogenized diverticula were differentially centrifuged in order to establish the distribution of types of lipides in the various cellular components. In addition, data have been presented which demonstrate a direct correlation between the titer of alpha-glycerol ethers and that of acetone-soluble lipide acetals in the unsaponifiable fraction.
已通过组织化学和生物化学方法对福布斯海盘车憩室的脂质进行了研究。对切片进行组织化学检查所获得的结果与对分离出的脂质进行化学分析的结果之间建立了相关性，特别是关于磷脂、类固醇和醛脂质方面。关于磷脂部分的性质、丙酮可溶性醛脂质的存在以及游离滴状脂肪的组成，组织化学研究的结果与化学数据高度吻合。为了确定各种脂质类型在不同细胞成分中的分布，对匀浆后的憩室进行了差速离心。此外，还提供了数据，这些数据表明在不皂化部分中α-甘油醚的滴度与丙酮可溶性脂质缩醛的滴度之间存在直接相关性。
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