Edy J, Collen D
Biochim Biophys Acta. 1977 Oct 13;484(2):423-32. doi: 10.1016/0005-2744(77)90098-5.
The inhibition of plasmin, (EC 3.4.21.7), thrombin (EC 3.4.21.5), trypsin (EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1) by antiplasmin, the recently described fast-reacting plasmin inhibitor of human plasma, was studied. To determine the quantitative importance of antiplasmin relative to the other plasma protease inhibitors, enzyme inhibition assays were performed on whole plasma and on plasma specifically depleted in antiplasmin, after addition of excess enzyme. Plasmin was the only enzyme for which the inhibitory capacity of antiplasmin-depleted plasma was lower than that of normal plasma. To determine the affinity of the enzymes for antiplasmin, as compared to the other inhibitors, various amounts of enzymes were added to normal plasma and the formation of enzyme-antiplasmin complexes studied by crossed immunoelectrophoresis using specific antisera against antiplasmin. Plasmin and trypsin, but not thrombin or chymotrypsin formed complexes with antiplasmin. It is concluded that antiplasmin is the only fast-reacting plasmin inhibitor of human plasma. It is also a fast-reacting inhibitor of trypsin but only accounts for a very small part of the fast-reacting trypsin-inhibitory activity of plasma. This can be explained by the low concentration of antiplasmin (1 muM) in normal plasma, compared to the other inhibitors (e.g. alpha1-antitrypsin: 40-80 muM).
研究了抗纤溶酶（最近描述的人血浆中快速反应的纤溶酶抑制剂）对纤溶酶（EC 3.4.21.7）、凝血酶（EC 3.4.21.5）、胰蛋白酶（EC 3.4.21.4）和胰凝乳蛋白酶（EC 3.4.21.1）的抑制作用。为了确定抗纤溶酶相对于其他血浆蛋白酶抑制剂的定量重要性，在加入过量酶后，对全血浆和特异性去除抗纤溶酶的血浆进行了酶抑制试验。纤溶酶是唯一一种抗纤溶酶去除血浆的抑制能力低于正常血浆的酶。为了确定与其他抑制剂相比，这些酶对抗纤溶酶的亲和力，向正常血浆中加入不同量的酶，并使用抗纤溶酶的特异性抗血清通过交叉免疫电泳研究酶 - 抗纤溶酶复合物的形成。纤溶酶和胰蛋白酶与抗纤溶酶形成复合物，而凝血酶和胰凝乳蛋白酶则不形成。结论是抗纤溶酶是人类血浆中唯一的快速反应纤溶酶抑制剂。它也是胰蛋白酶的快速反应抑制剂，但仅占血浆快速反应胰蛋白酶抑制活性的很小一部分。这可以通过正常血浆中抗纤溶酶的低浓度（1μM）与其他抑制剂（例如α1 - 抗胰蛋白酶：40 - 80μM）相比来解释。
