KLEINFELD R G, VON HAAM E
J Biophys Biochem Cytol. 1959 Dec;6(3):393-8. doi: 10.1083/jcb.6.3.393.
The effect of thioacetamide on dividing cells of regenerating rat liver has been studied. Rats were given daily subcutaneous injections of thioacetamide as a 1 per cent solution at a dosage of 5 mg./100 gm. body weight for 7 to 10 days, subjected to partial hepatectomy, and sacrificed 28 to 31 hours later. Thioacetamide treatment results in striking increases in the nuclear ribonucleoproteins of the liver cell without affecting the mitotic rate during regeneration (14). During mitosis, RNA-containing particles were seen within the spindle and coating the contracted chromosomes from prophase through metaphase or early anaphase. At telophase, prior to the reconstruction of the nuclear membrane, fine RNA-containing granules appeared within the compact chromosomal groups. These coalesced to form nucleoli corresponding in number to the number of nucleolar organizer regions. The nuclei and nucleoli showed a rapid increase in size during the reconstruction period when compared with corresponding figures of the control liver samples. Electron micrographs of interphase nucleoli indicated a similar basic granular structure in both drug-treated and control animals. The question is raised as to whether the increased nucleolar material merely made visible some of the nucleolar-chromosomal associations that normally occur in mitosis, or whether thioacetamide directly affects the synthetic activity of the contracted mitotic chromosomes.
已对硫代乙酰胺对再生大鼠肝脏分裂细胞的作用进行了研究。给大鼠每日皮下注射1%溶液形式的硫代乙酰胺，剂量为5毫克/100克体重，持续7至10天，然后进行部分肝切除术，并在28至31小时后处死。硫代乙酰胺处理导致肝细胞核糖核蛋白显著增加，而不影响再生过程中的有丝分裂率（14）。在有丝分裂期间，从前期到中期或早后期，纺锤体内可见含RNA的颗粒，且这些颗粒覆盖着收缩的染色体。在末期，在核膜重建之前，紧密染色体组内出现了细小的含RNA颗粒。这些颗粒聚合并形成数量与核仁组织区数量相对应的核仁。与对照肝脏样本的相应数据相比，在重建期细胞核和核仁大小迅速增加。间期核仁的电子显微镜照片显示，药物处理组和对照组动物的核仁基本颗粒结构相似。问题在于，核仁物质的增加仅仅是使有丝分裂中正常发生的一些核仁 - 染色体关联变得可见，还是硫代乙酰胺直接影响收缩的有丝分裂染色体的合成活性。
