KISSMEYER-NIELSEN F, MADSEN C B
J Clin Pathol. 1961 Nov;14(6):630-6. doi: 10.1136/jcp.14.6.630.
Platelet survival was determined using untreated and siliconed glass bottles and plastic bags (Fenwal) for collecting and storing blood. The platelets were tagged in vivo with P(32) in six polycythaemic patients undergoing treatment with P(32). The results showed that fresh ACD blood collected in untreated glass, siliconed glass, and plastic gave the same recovery of platelets in the recipients. The use of EDTA (Fenwal formula) as anticoagulant gave results inferior to those obtained with blood using ACD as anticoagulant. Even after storage up to 24 hours in untreated glass bottles (ordinary bank blood) a satisfactory recovery of platelets was observed. After storage for 72 hours the recovery was less but not negligible.
使用未处理的玻璃瓶、硅化玻璃瓶和塑料袋(芬华公司产品)收集和储存血液,以此测定血小板存活率。对6例接受³²P治疗的真性红细胞增多症患者体内的血小板用³²P进行标记。结果显示,用未处理的玻璃、硅化玻璃和塑料收集的新鲜ACD血在受血者体内的血小板回收率相同。使用乙二胺四乙酸(EDTA,芬华配方)作为抗凝剂得到的结果不如使用ACD作为抗凝剂的血液。即使在未处理的玻璃瓶中储存长达24小时(普通库存血),仍观察到血小板回收率令人满意。储存72小时后回收率降低,但并非微不足道。