Wallaert B, Voisin C
Laboratoire de Pollution Atmosphérique et de Pathologie Respiratoire Expérimentale, Institute Pasteur, Lille, France.
Cell Biol Toxicol. 1992 Jul-Sep;8(3):151-6. doi: 10.1007/BF00130522.
To evaluate the biological effects of gas pollutants on alveolar macrophages several in vitro systems have been developed. We described here an original method of cell culture in aerobiosis, which permitted direct contact between the atmosphere and the target cells. We studied the long term (24 h) and short term (30 min) effects of NO2 on alveolar macrophages. Our results demonstrated that exposure of alveolar macrophages to gas pollutants may be responsible for either cell injury or cell activation associated with the release of various bioactive mediators (superoxide anion, neutrophil chemotactic activity). Cell culture in aerobiosis opens new ways for the research on the biological effects of gas pollutants.
为评估气体污染物对肺泡巨噬细胞的生物学效应，已开发了多种体外系统。我们在此描述一种需氧条件下的细胞培养原始方法，该方法可使大气与靶细胞直接接触。我们研究了二氧化氮对肺泡巨噬细胞的长期（24小时）和短期（30分钟）效应。我们的结果表明，肺泡巨噬细胞暴露于气体污染物可能导致细胞损伤或与各种生物活性介质（超氧阴离子、中性粒细胞趋化活性）释放相关的细胞激活。需氧条件下的细胞培养为气体污染物生物学效应的研究开辟了新途径。
