使用荧光素酶报告基因检测法分析G12/13与G蛋白偶联受体的偶联情况。
Analysis of the coupling of G12/13 to G protein-coupled receptors using a luciferase reporter assay.
作者信息
Liu Bo, Wu Dianqing
机构信息
Department of Genetics and Developmental Biology, University of Connecticut, Farmington, USA.
出版信息
Methods Mol Biol. 2004;237:145-9. doi: 10.1385/1-59259-430-1:145.
G protein-coupled receptors (GPCRs) transduce signals from cell surface to intracellular effectors through various G proteins. In this chapter, a luciferase reporter gene assay system is described for the study of the coupling of GPCRs to G alpha 12/13. This assay system, in which ligand-stimulated production of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking G alpha q/11 that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to G alpha 12/13.
G蛋白偶联受体（GPCRs）通过各种G蛋白将细胞表面信号转导至细胞内效应器。在本章中，描述了一种荧光素酶报告基因检测系统，用于研究GPCRs与Gα12/13的偶联。该检测系统中，在缺乏Gαq/11的小鼠来源的细胞系中，在修饰的血清反应元件（SRE）控制下，报告荧光素酶的配体刺激产生，该细胞系转染了感兴趣的GPCR，从而能够轻松、快速且有效地确定GPCR与Gα12/13的偶联。
Zotero 插件