重新审视细菌核糖核酸酶P的底物识别：基于对底物中碱基N73的识别

Revisiting the substrate recognition of bacterial ribonuclease P: in the view of the recognition of the base N73 in the substrate.

作者信息

Tanaka Terumichi, Ando Tomoaki, Kikuchi Yo

机构信息

Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.

出版信息

Nucleic Acids Res Suppl. 2003(3):275-6. doi: 10.1093/nass/3.1.275.

DOI:10.1093/nass/3.1.275

PMID:14510487

Abstract

The RNA subunit of bacterial ribonuclease P (RNase P) is a ribozyme which can cleave a canonical cloverleaf tRNA precursor and a hairpin RNA with a CCA-3' tag sequence as its substrate. At high concentration of Mg ion, the substrate shape preference of the ribozyme becomes broader to accept a hairpin shape RNA. In hairpin RNA cleavage reactions, we found that the base interaction between the base U294 of E. coli ribozyme and the base N73 of the substrate RNA did not obey the response according to the Watson-Crick type interaction which is usually observed in the interaction between the base U294 of ribozyme and the base N73 of tRNA precursor.

摘要

细菌核糖核酸酶P（RNase P）的RNA亚基是一种核酶，它可以切割典型的三叶草型tRNA前体和带有CCA-3'标签序列的发夹RNA作为其底物。在高浓度镁离子存在下，核酶对底物形状的偏好变得更宽泛，能够接受发夹形状的RNA。在发夹RNA切割反应中，我们发现大肠杆菌核酶的碱基U294与底物RNA的碱基N73之间的碱基相互作用并不遵循通常在核酶的碱基U294与tRNA前体的碱基N73相互作用中观察到的沃森-克里克型相互作用的响应。