不同品种公鸡精液酸性磷酸酶和钙依赖性ATP酶活性与年龄的相关性。

Correlations between the activities of semen acid phosphatase and Ca(2+)-dependent ATPase and age in different breeds of cocks.

Dinischiotu A, Ion L, Dumitru I F

Department of Enzymology, University of Bucharest, Biological Faculty, Romania.

Comp Biochem Physiol B. 1992 Sep;103(1):289-92. doi: 10.1016/0305-0491(92)90446-x.

Abstract

The present work aims to find a biochemical criterion for evaluating the evolution of sperm according to age through the study of the ATPase activity from the spermatozoa and the acid phosphatase from the seminal plasma of cocks from three different breeds. 2. The optimal parameters of action of the cock semen acid phosphatase and the Ca(2+)-dependent ATPase from the spermatozoa were studied. 3. The substrate specificity of the semen acid phosphatase and its inhibition by tartrate, fluoride, metavanadate, molybdate and Hg2+ were also studied. 4. The two enzymes were determined from the Sussex, Golden Cornish and Plymouth Rock breeds at different ages. 5. The data lead to the conclusion that some properties of bird spermatozoa are less influenced by breed while the acid phosphatase activity, secreted in the ductus deferens is a breed characteristic.

摘要

本研究旨在通过对来自三个不同品种公鸡的精子中的ATP酶活性以及精浆中的酸性磷酸酶进行研究，找到一种根据年龄评估精子进化的生化标准。2. 研究了公鸡精液酸性磷酸酶和精子中钙依赖性ATP酶的最佳作用参数。3. 还研究了精液酸性磷酸酶的底物特异性及其受酒石酸盐、氟化物、偏钒酸盐、钼酸盐和Hg2+的抑制作用。4. 在不同年龄的苏塞克斯、金色康沃尔和普利茅斯洛克品种中测定了这两种酶。5. 数据得出结论，鸟类精子的某些特性受品种影响较小，而在输精管中分泌的酸性磷酸酶活性是品种特征。