Reyes Catherine D, García Andrés J
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
J Biomed Mater Res A. 2003 Oct 1;67(1):328-33. doi: 10.1002/jbm.a.10122.
A quantitative analysis of cell adhesion is essential in understanding physiological phenomena and designing biomaterials, implant surfaces, and tissue-engineering scaffolds. The most common cell adhesion assays used to evaluate biomaterial surfaces lack sensitivity and reproducibility and/or require specialized equipment and skill-intensive operation. We describe a modified centrifugation cell adhesion assay that uses simple and convenient techniques with standard laboratory equipment and provides reliable, quantitative measurements of cell adhesion. This centrifugation assay applies controlled and uniform detachment forces to a large population of adherent cells, providing robust statistics for quantifying cell adhesion. The applicability of this system to the design and characterization of biomaterial surfaces is shown by evaluating cell adhesion on substrates using different coating proteins, cell types, seeding times, and relative centrifugal forces (RCF). Results verify that this centrifugation cell adhesion assay represents a simple, convenient, and standard method for high-throughput characterization of a variety of biomaterial surfaces and conditions.
细胞黏附的定量分析对于理解生理现象以及设计生物材料、植入物表面和组织工程支架至关重要。用于评估生物材料表面的最常见细胞黏附测定方法缺乏灵敏度和可重复性，并且/或者需要专门的设备和技术密集型操作。我们描述了一种改良的离心细胞黏附测定方法，该方法使用简单便捷的技术和标准实验室设备，能够对细胞黏附进行可靠的定量测量。这种离心测定方法对大量贴壁细胞施加可控且均匀的脱离力，为量化细胞黏附提供了有力的统计数据。通过评估不同包被蛋白、细胞类型、接种时间和相对离心力（RCF）条件下细胞在底物上的黏附情况，展示了该系统在生物材料表面设计和表征方面的适用性。结果证实，这种离心细胞黏附测定方法是一种简单、便捷且标准的方法，可用于高通量表征各种生物材料表面和条件。
