Gal Tali Z, Glazer Itamar, Koltai Hinanit
Department of Nematology, ARO, The Volcani Center, Bet Dagan 50250, Israel.
J Parasitol. 2003 Aug;89(4):761-6. doi: 10.1645/GE-3105.
The developmentally arrested life stage of the entomopathogenic nematode Steinernema feltiae is exposed to threats of survival, including desiccation. We adopted a comprehensive approach to the study of the molecular mechanisms of desiccation stress tolerance in S. feltiae IS-6. We identified, expressed sequence tags (ESTs) that are differentially expressed during desiccation stress in S. feltiae IS-6 infective juveniles using DNA subtractive hybridization. These ESTs included genes that are known to be stress related, genes that are homologous to hypothetical Caenorhabditis elegans proteins, and novel genes that may be involved in traits specific to S. feltiae. Expression pattern characterization revealed that all analyzed ESTs were induced during 8 and 24 hr of dehydration of S. feltiae IS-6. Our results unveiled some of the components of the genetic networks that are activated in S. feltiae IS-6 during dehydration and suggested a differing pattern of temporal regulation during nematode dehydration.
昆虫病原线虫斯氏线虫发育停滞的生活阶段面临包括干燥在内的生存威胁。我们采用了一种综合方法来研究斯氏线虫IS-6对干燥胁迫耐受性的分子机制。我们利用DNA消减杂交技术，鉴定了斯氏线虫IS-6感染性幼虫在干燥胁迫期间差异表达的表达序列标签（ESTs）。这些ESTs包括已知与胁迫相关的基因、与秀丽隐杆线虫假定蛋白同源的基因，以及可能参与斯氏线虫特有性状的新基因。表达模式特征分析表明，所有分析的ESTs在斯氏线虫IS-6脱水8小时和24小时期间均被诱导。我们的结果揭示了斯氏线虫IS-6在脱水过程中被激活的遗传网络的一些组成部分，并表明线虫脱水期间存在不同的时间调控模式。
