Liu Lei, Lin Jun-Ji, Chen Xianhua, Liu Xuan, Xu Ping
Laboratory of Genomic Physiology and Center for Brain Research, School of Life Sciences, Fudan University, Shanghai, PR China.
Neuroreport. 2003 Oct 6;14(14):1847-50. doi: 10.1097/00001756-200310060-00018.
Neural salient serine-/arginine-rich protein 1 (NSSR1) is a newly identified SR protein that regulates pre-mRNA splicing. In the present study, we demonstrated the neural specialization of NSSR1 protein expression in humans and mice. Strong immunoreactive signals to NSSR1 were observed in mouse cerebral neurons, cerebellar Purkinje cells, pyramidal neurons in CA1, CA2 and CA3 regions of the hippocampus and granule cells in the dentate gyrus. In primarily cultured mouse neural progenitor cells (NPCs), at the undifferentiated status, NSSR1 transcripts were detected, but not the proteins. In comparison, in differentiated NPCs both NSSR1 transcripts and proteins were expressed and significantly up-regulated. The results suggest that NSSR1 is important in regulation of brain function and neural differentiation, possibly via regulating the neural-specific alternative splicing of genes.
神经显著富含丝氨酸/精氨酸蛋白1(NSSR1)是一种新发现的调节前体mRNA剪接的SR蛋白。在本研究中,我们证明了NSSR1蛋白在人和小鼠中的神经特异性表达。在小鼠脑神经元、小脑浦肯野细胞、海马CA1、CA2和CA3区的锥体细胞以及齿状回颗粒细胞中观察到对NSSR1的强免疫反应信号。在原代培养的小鼠神经祖细胞(NPC)中,处于未分化状态时,可检测到NSSR1转录本,但未检测到蛋白质。相比之下,在分化的NPC中,NSSR1转录本和蛋白质均有表达且显著上调。结果表明,NSSR1可能通过调节基因的神经特异性可变剪接,在脑功能调节和神经分化中起重要作用。