Zinn R A, Barrajas R, Montano M, Ware R A
Desert Research and Extension Center, University of California, El Centro 92243, USA.
J Anim Sci. 2003 Oct;81(10):2383-9. doi: 10.2527/2003.81102383x.
Four Holstein steers (282 kg) with cannulas in the rumen and proximal duodenum were used in a 4 x 4 Latin square experiment to evaluate the influence of dietary urea level (0, 0.4, 0.8, and 1.2%, DM basis) in a steam-flaked barley-based finishing diet on digestive function. There were no treatment effects (P > 0.20) on ruminal digestion of OM and ADF. Increasing dietary urea level increased (linear, P < 0.01) ruminal starch digestion. Ruminal degradability of protein in the basal diet (no supplemental urea) was 60%. Increasing dietary urea level did not increase (P > 0.20) ruminal microbial protein synthesis or nonammonia N flow to the small intestine. There were no treatment effects (P > 0.20) on total-tract ADF digestion. Total tract digestion of OM (quadratic, P < 0.01) and starch (linear, P < 0.05) increased slightly with increasing urea level. Urea supplementation increased (linear, P < 0.01) ruminal pH 1 h after feeding; however, by 3 h after feeding, ruminal pH was lower (cubic, P < 0.05) with urea-supplemented diets. Urea supplementation did not affect (P > 0.20) ruminal molar proportions of acetate and propionate. One hundred twenty crossbred steers (252 kg; approximately 25% Brahman breeding) were used in an 84-d feeding trial (five pens per treatment) to evaluate treatment effects on growth performance. Daily weight gain increased (linear, P = 0.01) with increasing urea level, tending to be maximal (1.53 kg/d; quadratic, P = 0.13) at the 0.8% level of urea supplementation. Improvements in ADG were due to treatment effects (linear, P < 0.01) on DMI. Urea supplementation did not affect (P > 0.20) the NE value of the diet for maintenance and gain. Observed dietary NE values, based on growth performance, were in close agreement with expected based on tabular values for individual feed ingredients, averaging 100.4%. We conclude that with steam-flaked barely-based finishing diets, ruminal and total-tract digestion of OM and ruminal microbial protein synthesis may not be increased by urea supplementation. In contrast, ADG was optimized by dietary inclusion of 0.8% urea. Urea supplementation may not enhance the net energy value of steam-flaked barely-based finishing diets when degradable intake protein is greater than 85% of microbial protein synthesis.
选用4头装有瘤胃和十二指肠近端套管的荷斯坦阉牛(体重282千克),进行4×4拉丁方试验,以评估蒸汽压片大麦育肥日粮中尿素水平(0、0.4、0.8和1.2%,干物质基础)对消化功能的影响。日粮处理对瘤胃中有机物(OM)和酸性洗涤纤维(ADF)的消化率没有显著影响(P>0.20)。随着日粮中尿素水平的增加,瘤胃淀粉消化率呈线性增加(P<0.01)。基础日粮(不添加尿素)中蛋白质的瘤胃降解率为60%。增加日粮中尿素水平并没有增加瘤胃微生物蛋白合成或非氨态氮向小肠的流量(P>0.20)。日粮处理对全消化道ADF消化率没有显著影响(P>0.20)。随着尿素水平的增加,全消化道OM消化率(二次效应,P<0.01)和淀粉消化率(线性效应,P<0.05)略有增加。添加尿素使采食后1小时瘤胃pH值呈线性增加(P<0.01);然而,采食后3小时,添加尿素的日粮瘤胃pH值较低(三次效应,P<0.05)。添加尿素对瘤胃乙酸和丙酸的摩尔比例没有影响(P>0.20)。选用120头杂种阉牛(体重252千克;约含25%婆罗门牛血统)进行为期84天的饲养试验(每个处理5个栏),以评估日粮处理对生长性能的影响。随着尿素水平的增加,日增重呈线性增加(P=0.01),在添加0.8%尿素水平时趋于最大(1.53千克/天;二次效应,P=0.13)。平均日增重(ADG)的提高归因于干物质采食量(DMI)的处理效应(线性效应,P<0.01)。添加尿素对日粮维持和增重的净能值没有影响(P>0.20)。根据生长性能观测到的日粮净能值与根据各饲料原料表值预期的净能值密切相符,平均为100.4%。我们得出结论,对于以蒸汽压片大麦为基础的育肥日粮,添加尿素可能不会增加瘤胃和全消化道对OM的消化率以及瘤胃微生物蛋白合成。相反,日粮中添加0.8%尿素可使ADG达到最佳。当可降解摄入蛋白大于微生物蛋白合成的85%时,添加尿素可能不会提高以蒸汽压片大麦为基础的育肥日粮的净能值。