大肠杆菌中前体导向的聚酮化合物生物合成

Precursor-Directed polyketide biosynthesis in Escherichia coli.

作者信息

Kinoshita Kenji, Pfeifer Blaine A, Khosla Chaitan, Cane David E

机构信息

Department of Chemistry, Box H, Brown University, Providence, RI 02912-9108, USA.

出版信息

Bioorg Med Chem Lett. 2003 Nov 3;13(21):3701-4. doi: 10.1016/j.bmcl.2003.08.008.

DOI:10.1016/j.bmcl.2003.08.008

PMID:14552761

Abstract

Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.

摘要

在异源宿主大肠杆菌中证实了前体导向的聚酮生物合成。将几种二酮和三酮底物加入到含有来自脱氧红霉内酯B合酶（DEBS）变体形式的重组大肠杆菌菌株中，该变体形式的第一个延伸模块被删除，结果二酮底物成功形成了大环内酯，但三酮底物未成功。