一种新型二糖——葡萄糖基1,5-脱水-D-果糖（1,5-脱水-3-O-α-D-吡喃葡萄糖基-D-果糖）的制备及反应活性

Preparation and reactivity of a novel disaccharide, glucosyl 1,5-anhydro-D-fructose (1,5-anhydro-3-O-alpha-glucopyranosyl-D-fructose).

作者信息

Yoshinaga Kazuhiro, Abe Jun-ichi, Tanimoto Toshiko, Koizumi Kyoko, Hizukuri Susumu

机构信息

The United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto 1-21-4, Kagoshima 890-0065, Japan.

出版信息

Carbohydr Res. 2003 Oct 10;338(21):2221-5. doi: 10.1016/s0008-6215(03)00341-0.

DOI:10.1016/s0008-6215(03)00341-0

PMID:14553983

Abstract

A novel disaccharide, glucosyl 1,5-anhydro-D-fructose (1,5-anhydro-3-O-alpha-glucopyranosyl-D-fructose, GAF) was enzymatically prepared from 1,5-anhydro-D-fructose (1,5-AF) and cyclomaltoheptaose (beta-cyclodextrin). Cyclodextrin glucanotransferase transferred various sizes of maltooligosaccharide to 1,5-AF. Glucoamylase digested the maltooligosyl chain of the products to a glucosyl residue giving a final product, GAF. An NMR analysis of GAF elucidated that the glucose residue was linked to C-3 of the 1,5-AF residue with an ether linkage. Reactivity on the aminocarbonyl reaction of GAF with bovine serum albumin was lower than that of 1,5-AF, but was higher than that of glucose.

摘要

一种新型二糖，葡萄糖基1,5-脱水-D-果糖（1,5-脱水-3-O-α-吡喃葡萄糖基-D-果糖，GAF）是由1,5-脱水-D-果糖（1,5-AF）和环麦芽七糖（β-环糊精）酶法制备而成。环糊精葡糖基转移酶将不同大小的麦芽寡糖转移至1,5-AF上。葡糖淀粉酶将产物的麦芽寡糖链消化为一个葡萄糖残基，从而得到最终产物GAF。对GAF的核磁共振分析表明，葡萄糖残基通过醚键与1,5-AF残基的C-3相连。GAF与牛血清白蛋白的氨基羰基反应活性低于1,5-AF，但高于葡萄糖。