Silva Juan, Aravena Carmen, Araya Jorge, Colque-Navarro Patricia, Kühn Inger, Möllby Roland
Departamento Tecnología Médica-INDES, Universidad de Antofagasta, Avenida Coloso s/n. Antofagasta, Chile.
Rev Med Chil. 2003 Aug;131(8):837-45.
PhP-S48 (Phene Plate Techniques AB), a method based on biochemical phenotypes has been developed and used successfully to typify S enteritidis strains in epidemiological studies.
To identify phenotypes of S enteritidis isolated from eggs, chicken meat and infected humans in Antofagasta during the period 1997-2000.
PhP-S48 and phage typing were used to identify phenotypes of 33 S enteritidis strains, sixteen isolated from poultry and 17 from clinical sources. S enteritidis ATCC17036 was used as control strain.
Twelve biochemical phenotypes (BTs) including 4 common (C) and 8 single (S) were identified. BTs C1 y C3 containing 16 and 5 strains, respectively, accounted for 63.6% of the isolates. BT C1 was found in poultry and human sources in the period 1997-2000, and BT C3 was isolated from humans, in the period 1999-2000. Using phage typing, 5 phage types (PT) and 3 strains could be not typed (NTs). PT1 and PT21 were the dominant phage types, with 14 and 13 strains respectively. Strains of PT1 were isolated from poultry and human sources in the period 1997-2000. PT21 was found in poultry samples in the period 1997-1998 and in clinical samples, in the period 1997-1998. Combination of biochemical phenotypes and phage typing divided the strains into 5 phenotypes (BT:PT). Two phenotypes were the most frequently isolated, phenotype C1:1 with 8 isolates found in eggs and humans in 1999, and phenotype C1:21 with 5 strains isolated in 1997-1999.
These results indicate the presence of one persistent and one recently emerged phenotype among S enteritidis in Antofagasta, Chile. PhP-S48 also provided information about a relationship among the strains.
PhP-S48（芬尼平板技术公司）是一种基于生化表型的方法，已被开发并成功用于在流行病学研究中对肠炎沙门氏菌菌株进行分型。
鉴定1997年至2000年期间在安托法加斯塔从鸡蛋、鸡肉和受感染人类中分离出的肠炎沙门氏菌的表型。
使用PhP-S48和噬菌体分型来鉴定33株肠炎沙门氏菌菌株的表型，其中16株从家禽中分离，17株从临床来源分离。肠炎沙门氏菌ATCC17036用作对照菌株。
鉴定出12种生化表型（BTs），包括4种常见（C）表型和8种单一（S）表型。BTs C1和C3分别包含16株和5株，占分离株的63.6%。BT C1在1997年至2000年期间在家禽和人类来源中发现，BT C3在1999年至2000年期间从人类中分离出来。使用噬菌体分型，可分为5种噬菌体型（PT），3株无法分型（NTs）。PT1和PT21是主要的噬菌体型，分别有14株和13株。PT1菌株在1997年至2000年期间从家禽和人类来源中分离出来。PT21在1997年至1998年期间在家禽样本中发现，在1997年至1998年期间在临床样本中发现。生化表型和噬菌体分型的组合将菌株分为5种表型（BT:PT）。两种表型最常被分离出来，表型C1:1在1999年在鸡蛋和人类中发现8株，表型C1:21在1997年至1999年期间分离出5株。
这些结果表明在智利安托法加斯塔的肠炎沙门氏菌中存在一种持续存在的表型和一种最近出现的表型。PhP-S48还提供了有关菌株之间关系的信息。
