药理学中分子相互作用的生物传感器分析

Biosensor profiling of molecular interactions in pharmacology.

作者信息

Cooper Matthew A

机构信息

Cambridge Centre for Molecular Recognition, University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, UK.

出版信息

Curr Opin Pharmacol. 2003 Oct;3(5):557-62. doi: 10.1016/j.coph.2003.05.003.

DOI:10.1016/j.coph.2003.05.003

PMID:14559103

Abstract

Techniques employed to profile the pharmacological properties of a molecule in vitro normally require some type of radio-, enzymatic- or fluorescent-labeling of the ligand and/or the receptor. In contrast, biosensor techniques do not require labeling, and they allow virtually any complex to be screened with minimal assay development. Scientists in both academia and industry are now using biosensors in areas that encompass almost all sectors of drug discovery, diagnostics and the life sciences. Assays have been developed for the analysis of small molecules, proteins, oligonucleotides, bacteriophage, viruses, bacteria and cells. In addition, novel biosensor applications are being developed for the predictive profiling of key pharmacokinetic parameters of a molecule (adsorption, distribution, metabolism, excretion and toxicity).

摘要

体外分析分子药理学特性所采用的技术通常需要对配体和/或受体进行某种类型的放射性、酶促或荧光标记。相比之下，生物传感器技术不需要标记，并且几乎可以在最少的分析开发情况下对任何复合物进行筛选。学术界和工业界的科学家目前都在几乎涵盖药物发现、诊断和生命科学所有领域的各个方面使用生物传感器。已经开发出用于分析小分子、蛋白质、寡核苷酸、噬菌体、病毒、细菌和细胞的检测方法。此外，正在开发新型生物传感器应用，用于预测分子关键药代动力学参数（吸收、分布、代谢、排泄和毒性）。