吡唑并[3,4-d]嘧啶核酸：dA-dT与dG-dC碱基对稳定性的调节。
Pyrazolo[3,4-d]pyrimidine nucleic acids: adjustment of the dA-dT to the dG-dC base pair stability.
作者信息
He J, Becher G, Budow S, Seela F
机构信息
Laboratorium für Organische und Bioorganische Chemie, Institut für Chemie, Universität Osnabrück, Osnabrück, Germany.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):573-6. doi: 10.1081/ncn-120021957.
The pyrazolo[3,4-d]pyrimidine-4,6-diamine nucleosides 2b-d stabilize the dA-dT base pair significantly when the dA-residue is replaced. Oligonucleotide duplexes incorporating 2b-d show a 4-6 degrees C Tm increase per modification. The 7-bromo compound 2b harmonizes the stability of the dA-dT vs. the dG-dC pair. According to this the stability of such duplexes depends no longer on the base pair composition of a DNA molecule.
当腺嘌呤（dA）残基被取代时，吡唑并[3,4-d]嘧啶-4,6-二胺核苷2b-d能显著稳定dA-dT碱基对。掺入2b-d的寡核苷酸双链体每修饰一处，其解链温度（Tm）会升高4-6摄氏度。7-溴化合物2b使dA-dT与dG-dC碱基对的稳定性趋于一致。据此，此类双链体的稳定性不再取决于DNA分子的碱基对组成。
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