人鳞状细胞癌A431细胞的细胞表面寡核苷酸结合蛋白

Cell surface oligonucleotide-binding proteins of human squamous carcinoma A431 cells.

作者信息

Laktionov P P, Chelobanov B P, Kharkova M V, Rykova E Yu, Pyshnyi D V, Pyshnaya I A, Marcus K, Meyer H E, Vlassov V V

机构信息

Group of Cell Biology, Novosibirsk Institute of Bioorganic Chemistry SB RAS, Novosibirsk, Russia. lakt.niboch.nsc

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1715-9. doi: 10.1081/NCN-120023121.

DOI:10.1081/NCN-120023121

PMID:14565503

Abstract

Affinity modified with Flu-DAP-p(N)16degU oligonucleotide-binding proteins were isolated by affinity chromatography using Ultrogel A2-anti fluorescein antibodies. After separation by SDS-PAGE the proteins with molecular masses about 68 kDa were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e and albumin.

摘要

用Flu-DAP-p(N)16degU寡核苷酸结合蛋白修饰的亲和物，通过使用Ultrogel A2 - 抗荧光素抗体的亲和色谱法进行分离。经SDS - PAGE分离后，对分子量约为68 kDa的蛋白质进行了串联质谱测序，并鉴定为角蛋白K1、角蛋白K10、角蛋白K2e和白蛋白。