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CdtA-CdtC复合物可阻断杜克雷嗜血杆菌细胞致死性膨胀毒素对HeLa细胞的杀伤作用。

A CdtA-CdtC complex can block killing of HeLa cells by Haemophilus ducreyi cytolethal distending toxin.

作者信息

Deng Kaiping, Hansen Eric J

机构信息

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9048, USA.

出版信息

Infect Immun. 2003 Nov;71(11):6633-40. doi: 10.1128/IAI.71.11.6633-6640.2003.

Abstract

The cytolethal distending toxin (CDT) of Haemophilus ducreyi is comprised of the CdtA, CdtB, and CdtC proteins, with the CdtB protein having demonstrated enzymatic (i.e., DNase) activity. Using a single recombinant Escherichia coli strain with two plasmids individually containing the H. ducreyi cdtA and cdtC genes, we purified a noncovalent CdtA-CdtC complex. Incubation of this CdtA-CdtC complex with HeLa cells blocked killing of these cells by CDT holotoxin. Furthermore, the addition of purified recombinant CdtB to HeLa cells preincubated with the CdtA-CdtC complex resulted in the killing of these human epithelial cells.

摘要

杜克雷嗜血杆菌的细胞致死性膨胀毒素(CDT)由CdtA、CdtB和CdtC蛋白组成,其中CdtB蛋白已显示出酶活性(即脱氧核糖核酸酶活性)。我们使用单个重组大肠杆菌菌株,该菌株带有两个分别含有杜克雷嗜血杆菌cdtA和cdtC基因的质粒,纯化了一种非共价的CdtA-CdtC复合物。将这种CdtA-CdtC复合物与HeLa细胞一起孵育可阻止这些细胞被CDT全毒素杀死。此外,将纯化的重组CdtB添加到预先与CdtA-CdtC复合物孵育的HeLa细胞中,会导致这些人上皮细胞死亡。

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本文引用的文献

1
Functional studies of the recombinant subunits of a cytolethal distending holotoxin.
Cell Microbiol. 2002 Apr;4(4):245-55. doi: 10.1046/j.1462-5822.2002.00186.x.
2
Immunopathogenesis of Haemophilus ducreyi infection (chancroid).
Infect Immun. 2002 Apr;70(4):1667-76. doi: 10.1128/IAI.70.4.1667-1676.2002.
3
Cytolethal distending toxin: limited damage as a strategy to modulate cellular functions.
Trends Microbiol. 2002 Mar;10(3):147-52. doi: 10.1016/s0966-842x(02)02316-8.
5
Cytolethal distending toxin demonstrates genotoxic activity in a yeast model.
Infect Immun. 2001 Sep;69(9):5752-9. doi: 10.1128/IAI.69.9.5752-5759.2001.
6
Characterization of Haemophilus ducreyi cdtA, cdtB, and cdtC mutants in in vitro and in vivo systems.
Infect Immun. 2001 Sep;69(9):5626-34. doi: 10.1128/IAI.69.9.5626-5634.2001.
7
Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans.
Microbiol Immunol. 2001;45(6):497-506. doi: 10.1111/j.1348-0421.2001.tb02650.x.
8
Investigation of the interaction among the components of the cytolethal distending toxin of Haemophilus ducreyi.
Biochem Biophys Res Commun. 2001 Jul 20;285(3):609-15. doi: 10.1006/bbrc.2001.5223.
9
CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity.
Infect Immun. 2001 Jul;69(7):4358-65. doi: 10.1128/IAI.69.7.4358-4365.2001.

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