Martín-Badosa E, Elmoutaouakkil A, Nuzzo S, Amblard D, Vico L, Peyrin F
ESRF (European Synchrotron Radiation Facility), BP 220, 38043 Grenoble, France.
Comput Med Imaging Graph. 2003 Nov-Dec;27(6):447-58. doi: 10.1016/s0895-6111(03)00031-4.
We developed an automatic method to characterize mice bone architecture from three-dimensional (3D) microtomographic images. The distal metaphyses of the femur of mice were imaged using 3D synchrotron radiation microtomography at the European Synchrotron Radiation Facility (ID19) with a voxel size of 6.65 mum. Within each reconstructed volume, a region of interest was defined and trabecular and cortical bones were automatically separated. Then, 3D morphologic and topologic model-independent parameters quantifying the 3D bone architecture were computed in both regions. The technique was applied to study the response of the C57BL/6J@Ico strain of mice submitted to a model of bone loss by hind limb unloading produced by tail-suspension.
我们开发了一种自动方法，用于从三维（3D）显微断层图像中表征小鼠骨骼结构。在欧洲同步辐射装置（ID19）使用3D同步辐射显微断层扫描对小鼠股骨远端干骺端进行成像，体素大小为6.65微米。在每个重建体积内，定义一个感兴趣区域，并自动分离小梁骨和皮质骨。然后，在两个区域计算量化3D骨骼结构的3D形态学和拓扑学独立于模型的参数。该技术应用于研究C57BL/6J@Ico品系小鼠在尾吊产生的后肢卸载导致的骨质流失模型中的反应。
