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急性肾移植排斥反应期间CD4 T细胞上CD45表达的下调:T抑制/诱导活性下降的证据。

Down regulation of CD45 expression on CD4 T cells during acute renal allograft rejection: evidence of a decline in T suppressor/inducer activity.

作者信息

Shabtai M, Waltzer W C, Ayalon A, Shabtai E L, Malinowski K

机构信息

Department of Surgery and Transplantation, Sheba Medical Center, Tel Aviv University School of Medicine, Tel Hashomer, 52621, Israel.

出版信息

Int Urol Nephrol. 2002;34(4):555-8. doi: 10.1023/a:1025641404743.

Abstract

Acute rejection is associated with the activation of helper and cytotoxic cells. A shifting balance between the suppressor/inducer CD45+ CD4+ and T helper/inducer (CD4+CD45-) cells may be responsible for the transition from quiescence to overt rejection. We examined the kinetics of CD45 expression on CD4+ T cells in renal allograft recipients from pretransplant values to acute rejection and after reversal of rejection, searching for a shift in balance between helper/inducer and suppressor/inducer cell subsets. Using two color flow cytometry, the peripheral blood levels of CD4+, CD4+CD45- [T helper/inducer (Thi)], CD4+CD45+ [T suppressor/inducer (Tsi)], CD3+, and CD8+ T cells subsets and their interrelationships, were determined in 49 patients prior to transplantation, and in 10 of them, during acute rejection and after its reversal. Results were analyzed and compared to data obtained from 10 healthy blood donors. Acute rejection was associated with a significant decline in CD45+ CD4+ expression compared to quiescent phase (22% +/- 3.7% vs. 26.5% +/- 3.2%, p = 0.05) and controls (29.5% +/- 6.2%, p = 0.01). No difference was observed compared to pretransplant levels (19.9% +/- 3.2%, p = ns). CD45-/CD45+ (Thi/Tsi) ratio was lowest during quiescence (0.75) compared to rejection (0.97, p = 0.05), in controls (0.98, p = 0.05) and pretransplant values (1.4, p = 0.01). Acute rejection was characterized by higher Thi/CD8+ and lower Tsi/CD8+ ratio (103 and 88 respectively, p = 0.045), compared to clinical quiescence (104 and 116 respectively, p = 0.039). These data suggest that acute rejection is associated with down regulation of CD4+CD45+ suppressor/inducer subset. This shift may account for the transition from quiescence to overt rejection, concurring with reports on CD4+CD45 regulatory function.

摘要

急性排斥反应与辅助性细胞和细胞毒性细胞的激活相关。抑制/诱导性CD45+ CD4+细胞与辅助性/诱导性T细胞(CD4+CD45-)之间平衡的改变可能是导致从静止状态转变为明显排斥反应的原因。我们研究了肾移植受者中CD4+ T细胞上CD45表达的动力学,从移植前到急性排斥反应以及排斥反应逆转后,寻找辅助性/诱导性和抑制性/诱导性细胞亚群之间平衡的变化。使用双色流式细胞术,测定了49例患者移植前外周血中CD4+、CD4+CD45- [辅助性/诱导性T细胞(Thi)]、CD4+CD45+ [抑制性/诱导性T细胞(Tsi)]、CD3+和CD8+ T细胞亚群及其相互关系,其中10例患者在急性排斥反应期间及逆转后也进行了测定。对结果进行分析,并与10名健康献血者的数据进行比较。与静止期相比(22%±3.7%对26.5%±3.2%,p = 0.05)以及与对照组相比(29.5%±6.2%,p = 0.01),急性排斥反应与CD45+ CD4+表达的显著下降相关。与移植前水平相比(19.9%±3.2%,p =无显著差异)未观察到差异。与排斥反应(0.97,p = 0.05)、对照组(0.98,p = 0.05)和移植前水平(1.4,p = 0.01)相比,静止期的CD45-/CD45+(Thi/Tsi)比值最低(0.75)。与临床静止期相比(分别为104和116,p = 0.039),急性排斥反应的特征在于Thi/CD8+比值较高,Tsi/CD8+比值较低(分别为103和88,p = 0.045)。这些数据表明,急性排斥反应与CD4+CD45+抑制性/诱导性亚群的下调相关。这种转变可能解释了从静止状态到明显排斥反应的转变,这与关于CD4+CD45调节功能的报道一致。

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