Hansen J L, Etchison D O, Hershey J W, Ehrenfeld E
Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, Utah 84132, USA.
Mol Cell Biol. 1982 Dec;2(12):1639-43. doi: 10.1128/mcb.2.12.1639-1643.1982.
The 26,000-M(r) cap-binding protein was analyzed by a cross-linking assay in cell fractions from uninfected and poliovirus-infected HeLa cells. Cap-binding protein was found in the postribosomal supernatant (S-200) and in the ribosomal salt wash. The cap-binding protein in the S-200 had a sedimentation coefficient of 5 to 7S and lacked the ability to restore translation in extracts of poliovirus-infected cells.
通过交联分析对来自未感染和脊髓灰质炎病毒感染的HeLa细胞的细胞组分中的26,000-M（r）帽结合蛋白进行了分析。在核糖体后上清液（S-200）和核糖体盐洗物中发现了帽结合蛋白。S-200中的帽结合蛋白沉降系数为5至7S，并且缺乏恢复脊髓灰质炎病毒感染细胞提取物中翻译的能力。
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