Zhang Zhongqiu, Futamura Manabu, Vikis Haris G, Wang Min, Li Jie, Wang Yian, Guan Kun-Liang, You Ming
Department of Surgery and The Alvin J. Siteman Cancer Center, Washington University School of Medicine, Campus Box 8109, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12642-7. doi: 10.1073/pnas.2133947100.
Pulmonary adenoma susceptibility 1 (Pas1), located on chromosome 6, is the major locus affecting inherited predisposition to lung tumor development in mice. We have fine mapped the Pas1 locus to a region of approximately 0.5 megabases by using congenic strains of mice, constructed by placing the Pas1 region of chromosome 6 from A/J mice onto the genetic background of C57BL/6J mice. Systematic characterization of Pas1 candidates establishes the Las1 (lung adenoma susceptibility 1) and Kras2 (Kirsten rat sarcoma oncogene 2) genes as primary candidates for the Pas1 locus. Clearly, Kras2 affects lung tumor progression only, and Las1 is likely to affect lung tumor multiplicity.
肺腺瘤易感性1(Pas1)位于6号染色体上,是影响小鼠肺癌发生遗传易感性的主要基因座。我们通过构建同源近交系小鼠,将A/J小鼠6号染色体的Pas1区域置于C57BL/6J小鼠的遗传背景上,从而将Pas1基因座精细定位到大约0.5兆碱基的区域。对Pas1候选基因的系统表征确定了Las1(肺腺瘤易感性1)和Kras2( Kirsten大鼠肉瘤癌基因2)基因为Pas1基因座的主要候选基因。显然,Kras2仅影响肺肿瘤进展,而Las1可能影响肺肿瘤的多发性。