Simon R
Biometric Research Branch, Division of Cancer Treatment & Diagnosis, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, MSC #7434, Bethesda, MD 20892, USA.
Br J Cancer. 2003 Nov 3;89(9):1599-604. doi: 10.1038/sj.bjc.6601326.
DNA microarrays are a potentially powerful technology for improving diagnostic classification, treatment selection and therapeutics development. There are, however, many potential pitfalls in the use of microarrays that result in false leads and erroneous conclusions. This paper provides a review of the key features to be observed in developing diagnostic and prognostic classification systems based on gene expression profiling and some of the pitfalls to be aware of in reading reports of microarray-based studies.
DNA微阵列是一种在改善诊断分类、治疗选择和治疗方法开发方面具有潜在强大功能的技术。然而,在使用微阵列时存在许多潜在的陷阱,这些陷阱会导致错误的线索和错误的结论。本文综述了基于基因表达谱开发诊断和预后分类系统时应注意的关键特征,以及在阅读基于微阵列研究的报告时应注意的一些陷阱。