大鼠胫骨中甘油醛-3-磷酸脱氢酶稳态mRNA水平的意外变化。
Unanticipated changes in steady-state mRNA levels for glyceraldehyde-3-phosphate dehydrogenase in rat tibiae.
作者信息
Maran A, Hefferan T E, Zhang M, Turner R T
机构信息
Dept. of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
出版信息
Calcif Tissue Int. 2004 Feb;74(2):204-7. doi: 10.1007/s00223-002-1098-2. Epub 2003 Nov 3.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels are commonly used as an internal control to normalize gene expression data based on the belief that this gene is constitutively expressed. However, GAPDH mRNA levels increased by more than 2.5-fold in tibiae of hindlimb unloaded female rats compared to L32 mRNA levels. Similarly, GAPDH mRNA levels increased compared to 18S ribosomal RNA. Treatment with growth hormone and alcohol show no disparity in GAPDH mRNA levels whereas in some experiments, parathyroid hormone and 17beta-estradiol increased GAPDH mRNA levels. Taken together, these findings indicate that it is essential to demonstrate that GAPDH expression is not altered prior to using the gene as an internal control.
甘油醛-3-磷酸脱氢酶（GAPDH）的mRNA水平通常被用作内参来标准化基因表达数据，这是基于该基因组成性表达的观点。然而，与L32 mRNA水平相比，后肢卸载雌性大鼠胫骨中GAPDH mRNA水平增加了2.5倍以上。同样，与18S核糖体RNA相比，GAPDH mRNA水平也有所增加。生长激素和酒精处理后，GAPDH mRNA水平没有差异，而在一些实验中，甲状旁腺激素和17β-雌二醇可增加GAPDH mRNA水平。综上所述，这些发现表明，在将该基因用作内参之前，必须证明GAPDH的表达没有改变。
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