用大口径毛细管气相色谱法快速分析烟草及卷烟烟雾中与尼古丁相关的生物碱
Fast analysis of nicotine related alkaloids in tobacco and cigarette smoke by megabore capillary gas chromatography.
作者信息
Cai Jibao, Liu Baizhan, Lin Ping, Su Qingde
机构信息
Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
出版信息
J Chromatogr A. 2003 Oct 31;1017(1-2):187-93. doi: 10.1016/j.chroma.2003.07.003.
A novel fast megabore capillary gas chromatographic (MCGC) method for analysis of 7 nicotine related alkaloids in tobacco and cigarette smoke, including nicotine, nornicotine, myosmine, nicotyrine, anabasine, anatabine and 2,3-dipyridyl, was developed. The use of megabore capillary column GC methodology, equipped with flame ionization detector (FID), provided rapid, unambiguous nicotine related alkaloids analysis. One gram flue-cured tobacco (or Cambridge filter pad), 20 ml ether, and 5 ml 10% sodium hydroxide solution, added with n-heptadecane as the internal standard, were placed in a flask, and the flask was capped and placed in an ultrasonic bath for 15 min. A 1 microl volume was analyzed by capillary GC operating in split-injection mode on a mega bore Simplicity-5 column. This simple procedure was compared with the previously reported packed column GC method and the Griffith still-colorimetric method. The application of the method for analysis of various flue-cured tobaccos and cigarette smoke was discussed.
开发了一种新型快速大口径毛细管气相色谱（MCGC）方法，用于分析烟草和卷烟烟雾中的7种与尼古丁相关的生物碱，包括尼古丁、降烟碱、麦斯明、烟碱、新烟草碱、假木贼碱和2,3-联吡啶。使用配备火焰离子化检测器（FID）的大口径毛细管柱气相色谱方法，能够快速、准确地分析与尼古丁相关的生物碱。将1克烤烟（或剑桥滤片）、20毫升乙醚和5毫升10%氢氧化钠溶液，加入正十七烷作为内标，置于烧瓶中，盖上瓶盖并放入超声浴中15分钟。取1微升体积的样品，通过在大口径Simplicity-5柱上以分流进样模式运行的毛细管气相色谱进行分析。将此简单方法与先前报道的填充柱气相色谱法和格里菲斯比色法进行了比较。讨论了该方法在分析各种烤烟和卷烟烟雾中的应用。
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