[用于鉴定微小脲原体和解脲脲原体的多重聚合酶链反应方法的开发]

[Development of a multiple PCR method for the identification of Ureaplasma parvum and Ureaplasma urealyticum].

作者信息

Fernández Molina C, Latino M A, Zamora Martínez Y, Pellecchia M, Neve V, Llanes R, Macfarlane R, Balbo L

机构信息

Instituto de Medicina Tropical Pedro Kourí, A.P. 601, Mariano 13, La Lisa, Ciudad de La Habana, Cuba.

出版信息

Rev Argent Microbiol. 2003 Jul-Sep;35(3):138-42.

PMID:14587375

Abstract

Ureaplasma parvum and Ureaplasma urealyticum, also known as biovar parvum and biovar T960, respectively, could be associated with several disorders in men, women, and mainly, in newborn children with under weight. Several methods have been developed in order to identify the species or biovars of ureaplasmas. We developed a Multiplex-PCR method using the UPS-UPSA and UUS2-UUA2 primers, specific for U. parvum and U. urealyticum, respectively. This Multiplex-PCR method was used to identify cultures of clinical positive samples to Ureaplasma spp. by the "MYCOFAST Evolution-2" Kit. Of 56 positive cultures to Ureaplasma spp. from newborn children, 70% were U. parvum and 30% U. urealyticum; in 76 positive samples in women, 83% corresponded to U. parvum and 17% to U. urealyticum, while in 63 positive samples of men, 76% identified U. parvum and 24% U. urealyticum. The PCR-multiplex method showed specificity for the identification of the biovars or species of ureaplasmas of clinical interest.

摘要

微小脲原体和解脲脲原体，也分别被称为生物变种微小脲原体和生物变种T960，可能与男性、女性，尤其是低体重新生儿的多种疾病有关。为了鉴定脲原体的种类或生物变种，已经开发了几种方法。我们使用分别针对微小脲原体和解脲脲原体的UPS-UPSA和UUS2-UUA2引物开发了一种多重PCR方法。这种多重PCR方法被用于通过“MYCOFAST Evolution-2”试剂盒鉴定脲原体属临床阳性样本的培养物。在56份新生儿脲原体属阳性培养物中，70%为微小脲原体，30%为解脲脲原体；在76份女性阳性样本中，83%对应微小脲原体，17%对应解脲脲原体，而在63份男性阳性样本中，76%鉴定为微小脲原体，24%为解脲脲原体。多重PCR方法显示出对鉴定临床相关脲原体生物变种或种类的特异性。