采用颗粒凝集试验对尼泊尔患者进行日本脑炎的血清学诊断。
Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay.
作者信息
Pandey B, Yamamoto A, Morita K, Kurosawa Y, Rai S, Adhikari S, Kandel P, Kurane I
机构信息
Department of Molecular Epidemiology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
出版信息
Epidemiol Infect. 2003 Oct;131(2):881-5. doi: 10.1017/s0950268803008835.
Japanese encephalitis (JE) is a serious health problem in the southwestern region of Nepal. Serological diagnostic kits for routine diagnostic use in this region have not been available. This study was performed to examine if the particle agglutination (PA) assay for Japanese encephalitis virus (JEV) IgM could be applicable to the samples collected in Nepal and also to evaluate the accuracy of clinical diagnosis of JE. One hundred and ninety-three blood samples were collected from the patients clinically diagnosed with JE or other infectious diseases in the JE-endemic, southwestern region of Nepal, in 2000. The PA assay was performed on these 193 serum samples and the results were compared with those by IgM-capture ELISA. Eighty-six samples were IgM-positive by the PA assay, and 71 of 86 were also positive by IgM-capture ELISA (sensitivity, 99%; specificity, 88%; positive predictive value, 0.82; negative predictive value, 0.99). These results suggest that the PA assay is a simple, reliable and useful diagnostic test to support clinical diagnosis in rural hospitals of Asia including Nepal.
日本脑炎（JE）在尼泊尔西南部地区是一个严重的健康问题。该地区尚无用于常规诊断的血清学诊断试剂盒。本研究旨在检验日本脑炎病毒（JEV）IgM的颗粒凝集（PA）试验是否适用于在尼泊尔采集的样本，并评估JE临床诊断的准确性。2000年，从尼泊尔西南部日本脑炎流行地区临床诊断为JE或其他传染病的患者中采集了193份血样。对这193份血清样本进行了PA试验，并将结果与IgM捕获ELISA的结果进行了比较。PA试验有86份样本IgM呈阳性，其中86份中的71份IgM捕获ELISA也呈阳性（敏感性99%；特异性88%；阳性预测值0.82；阴性预测值0.99）。这些结果表明，PA试验是一种简单、可靠且有用的诊断试验，可用于支持包括尼泊尔在内的亚洲农村医院的临床诊断。
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